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KMID : 1134820130420091419
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Journal of the Korean Society of Food Science and Nutrition
2013 Volume.42 No. 9 p.1419 ~ p.1425
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Method Validation for the Determination of Eleutherosidesand β-Glucan in Acanthopanax koreanum
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Kim Young-Hyun
Bae Da-Bin
Park Sun-Ok
Lee Sang-Jong
Cho Ok-Hyun
Lee Ok-Hwan
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Abstract
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The aim of this study was to investigate the method validation for the determination of eleutherosides (B and E) and β-glucan in Acanthopanax (A.) koreanum. This medicinal plant reportedly mainly included eleutherosides which exhibit the pharmacological effects, and β-glucan substantially enhances the function of the immune system by activating macrophages. The specificity, linearity, precision, accuracy, limit of detection (LOD, S/N=3), and limit of quantification (LOQ, S/N=10) were measured by HPLC and enzymatic methods. Our results showed that the coefficient of calibration correlation (R2) for eleutheroside B and E were 0.9997 and 0.9999, respectively. The limits of detection (LOD) for eleutheroside B and E were 0.050 μg/mL and 0.025 μg/mL, respectively. The recovery rate of eleutheroside B and E were revealed in the high range of 100.66～110.04% and 94.26～111.62%, respectively. The inter-day precision of eleutheroside B and E in the root and stem in A. koreanum were 1.4～5.0% and 1.1～2.5%, respectively. The intra-day precision of eleutheroside B and E in the root and stem in A. koreanum were 2.8～2.9% and 0.4～1.1%, respectively. Furthermore, the inter-day and intra-day precision of β-glucan in the stem, leaf, and fruit of A. koreanum were 1.32～5.67% and 8.01～11.76%, respectively. In conclusion, the methods were validated for the detection of eleutherosides and β-glucan in A. koreanum.
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KEYWORD
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Acanthopanax koreanum, eleutheroside B, eleutheroside E, β-glucan, method validation
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